The ban that has been in place since September on evening activities in City Parks has been lifted following the hard frost over the weekend.
"The Marlborough Board of Health has lifted the night-time restriction on outdoor activity in city parks due to lowered risk of West Nile Virus and EEE in the area," according to an announcement on the city's website.
The ban went into effect due to the risk of Eastern Equine Encephalitis (EEE) and after West Nile Virus (WNV) was discovered in Marlborough in August.
The Board of Health encourages residents to continue to use enhanced precautions with the use of Bug Spray containing DEET and wear long sleeves and long pants from dusk until dawn.
Tim Deschamps, the executive director of the Central Massachusetts Mosquito Control Project, had previously said that the threat of mosquito-borne illness would remain until the first or second frost of the season.